Russia lawmakers vote to annex Crimea; U.S. steps up sanctions俄罗斯国家杜马通过克里米亚入俄决议，美升格制裁。
Kiev (CNN) -- Russia's lower house of parliament overwhelmingly approved a treaty Thursday to annex the Black Sea peninsula of Crimea from Ukraine, prompting tougher sanctions from the United States.
Russia responded with its own sanctions against a list of U.S. officials and lawmakers.
After Russian Foreign Minister Sergey Lavrov had presented the treaty and urged lawmakers to accept the region as a part of the Russian Federation, the document was approved on a vote of 443 to 1.
Russia's Federation Council, the upper house of the parliament, will hold a similar vote Friday, completing ratification of a treaty that was signed by President Vladimir Putin on Tuesday.
Speaking "on behalf" of Putin, Lavrov had told the State Duma that folding Crimea into Russia was needed to protect ethnic Russians there.
"I am certain that passing the document will become a turning point in the destiny of multi-ethnic nations of Crimea and Russia, who are related with close ties of the historical unity," Lavrov said.
Russia's moves to annex Crimea have turned a confrontation with Europe and the United States into the biggest crisis in East-West relations since the Cold War.
Approval of the treaty in the State Duma was in no doubt as Russia has stood defiant despite Western leaders denouncing Moscow's actions as a violation of Ukraine's sovereignty and a breach of international law.
European Union leaders said Thursday they will sign a political association agreement with Ukraine and add 12 more people to the list of individuals targeted for sanctions.
EU member states also are threatening possible tougher targeted measures if Russia escalates the situation in Ukraine, European Council President Herman Van Rompuy told reporters.
"We all must remain cool-headed," he said.
U.S. and EU officials had already imposed sanctions on more than two dozen Russian and Crimean officials, and urged Russia to avoid escalating the crisis -- a call Moscow has ignored.
But U.S. President Barack Obama on Thursday announced more sanctions on individuals and one bank in response to Russia's annexation moves.
He also signed a new executive order that authorizes possible further sanctions on what he called "key sectors" of the Russian economy if Moscow does not act to deescalate the situation.
"This is not our preferred outcome. These sanctions would not only have a significant impact on the Russian economy, but could also be disruptive to the global economy," he said. "However Russia must know that further escalation will only isolate it further from the international community."
Russia must respect "basic principles" of sovereignty and territorial integrity, he said, adding that the United States should also provide financial support for Ukraine's government and people.
"We want the Ukrainian people to determine their own destiny and have good relations with the United States, Russia, Europe -- anyone they choose," he said, calling for continued diplomatic efforts.
The new U.S. sanctions target 20 officials, including senior Russians and "cronies" who hold significant influence in the Russian system, as well as one bank that holds "significant" resources, a senior U.S. administration official told reporters.
That bank was listed by the U.S. Treasury Department as Bank Rossiya.
The individuals named by the Treasury include major Putin allies, both in the Kremlin and in business. Among the 16 government officials listed are Putin's chief of staff, Sergei Ivanov; the speaker of the State Duma, Sergey Naryshkin; and Viktor Ozerov, chairman of the Security and Defense Committee of the Russia parliament's upper house.
Four others were named as members of the government's inner circle. They are financier Yuri Kovalchuk, labeled Putin's personal banker by a senior U.S. administration official; magnate Gennady Timchenko, whose activities in the energy sector have been directly linked to Putin, according to the Treasury; and businessmen Arkady and Boris Rotenberg.
Swedish Foreign Minister Carl Bildt told CNN's Christiane Amanpour that the new round of U.S. sanctions would be "significantly more powerful than the first one."
The latest round "hits significant economic interests that are fairly close to the ruling circles in Moscow. It will be noticed," he said.
Russia responded with sanctions against nine U.S. officials and lawmakers, including speaker of the U.S. House of Representatives John Boehner, Senate Majority leader Harry Reid and Sens. John McCain, Robert Menendez, Daniel Coats and Mary Landrieu, according to a list published by the Russian Foreign Ministry.
Boehner's spokesman, Michael Steel, said the lawmaker was "proud to be included on a list of those willing to stand against Putin's aggression."
McCain responded, "I guess this means my spring break in Siberia is off, my Gazprom stock is lost, and my secret bank account in Moscow is frozen. Nonetheless, I will never cease my efforts on behalf of the freedom, independence, and territorial integrity of Ukraine, including Crimea."
The U.S. Treasury said Bank Rossiya is controlled by Yuri Kovalchuk and is the 17th-largest bank in Russia.
It has $10 billion in assets and handles the accounts of some top government officials, the Treasury said, adding that the bank has relationships with banks in the United States, Europe, and elsewhere. The bank also provides services to the oil, gas and energy sectors, it said.
"As a result of Treasury's action, any assets of the persons designated today that are within U.S. jurisdiction must be frozen," the Treasury said.
British Prime Minister David Cameron told reporters at the EU Heads of State or Government summit there would likely be more asset freezes and travel bans.
Finland's Minister for European Affairs and Foreign Trade Alexander Stubb told CNN more names would be added Thursday.
"There will probably be over 10 new names on the list and then of course people are going to argue, are these people good to be on the list, bad to be on the list are they to be taken seriously and so on, but there will be more names," Stubb said.
Lavrov told lawmakers that sanctions "have never brought any positive results" and that there were no legal grounds for them.